Morphine-mediated release of miR-138 in astrocyte-derived extracellular vesicles promotes microglial activation.

ABSTRACT: Opioids, such as morphine, are the mainstay for the management of postsurgical pain. Over the last decade there has been a dramatic increase in deaths related to opioid overdose. While opioid abuse has been shown to result in increased neuroinflammation, mechanism(s) underlying this process, remain less understood. In recent years, microRNAs have emerged as key mediators of gene expression regulating both paracrine signaling and cellular crosstalk. MiRNAs constitute the extracellular vesicle (EV) cargo and can shuttle from the donor to the recipient cells. Exposure of human primary astrocytes to morphine resulted in induction and release of miR-138 in the EVs isolated from conditioned media of cultured astrocytes. Released EVs were, in turn, taken up by the microglia, leading to activation

SUBMITTER: Liao K 

PROVIDER: S-EPMC7710131 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature