Identification of Microorganisms by Liquid Chromatography-Mass Spectrometry (LC-MS¹) and in Silico Peptide Mass Libraries.

ABSTRACT: Over the past decade, modern methods of MS (MS) have emerged that allow reliable, fast and cost-effective identification of pathogenic microorganisms. Although MALDI-TOF MS has already revolutionized the way microorganisms are identified, recent years have witnessed also substantial progress in the development of liquid chromatography (LC)-MS based proteomics for microbiological applications. For example, LC-tandem MS (LC-MS²) has been proposed for microbial characterization by means of multiple discriminative peptides that enable identification at the species, or sometimes at the strain level. However, such investigations can be laborious and time-consuming, especially if the experimental LC-MS² data are tested against sequence databases covering a broad panel of dif

SUBMITTER: Lasch P 

PROVIDER: S-EPMC7710138 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature