Ontology highlight
ABSTRACT:
SUBMITTER: Zeng C
PROVIDER: S-EPMC7710271 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Zeng Cong C Evans John P JP Pearson Rebecca R Qu Panke P Zheng Yi-Min YM Robinson Richard T RT Hall-Stoodley Luanne L Yount Jacob J Pannu Sonal S Mallampalli Rama K RK Saif Linda L Oltz Eugene E Lozanski Gerard G Liu Shan-Lu SL
JCI insight 20201119 22
Rapid and specific antibody testing is crucial for improved understanding, control, and treatment of COVID-19 pathogenesis. Herein, we describe and apply a rapid, sensitive, and accurate virus neutralization assay for SARS-CoV-2 antibodies. The assay is based on an HIV-1 lentiviral vector that contains a secreted intron Gaussia luciferase (Gluc) or secreted nano-luciferase reporter cassette, pseudotyped with the SARS-CoV-2 spike (S) glycoprotein, and is validated with a plaque-reduction assay us ...[more]