Dataset Information

Genome-Wide Identification and Expression Pattern Analysis of the HAK/KUP/KT Gene Family of Cotton in Fiber Development and Under Stresses.

ABSTRACT: The potassium transporter family HAK/KUP/KT is a large group of proteins that are important in plant potassium transport and plays a crucial role in plant growth and development, especially in economic crops. Although HAK/KUP/KT genes have been identified in many species, research on these genes in cotton is still quite rare. In this study, in total, 21, 24, 45, and 44 HAK/KUP/KT genes were identified in Gossypium arboreum, Gossypium raimondii, Gossypium hirsutum, and Gossypium barbadense, respectively. Phylogenetic analysis showed that these genes were divided into four clusters. The G. hirsutum gene promoters contained diverse cis-regulatory elements, such as drought-responsive elements, low temperature-responsive elements, and other elements. The RNA-seq data and qRT-PCR results showed that HAK/KUP/KT genes had different expression patterns in fiber development. The qRT-PCR results of drought and NaCl treatment indicated that HAK/KUP/KT genes might play important roles in abiotic stress responses. These results will provide molecular insights into potassium transporter research in cotton.

SUBMITTER: Yang X

PROVIDER: S-EPMC7710864 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Genome-Wide Identification and Expression Pattern Analysis of the HAK/KUP/KT Gene Family of Cotton in Fiber Development and Under Stresses.

Yang Xu X Zhang Jingjing J Wu Aimin A Wei Hengling H Fu Xiaokang X Tian Miaomiao M Ma Liang L Lu Jianhua J Wang Hantao H Yu Shuxun S

Frontiers in genetics 20201119

The potassium transporter family HAK/KUP/KT is a large group of proteins that are important in plant potassium transport and plays a crucial role in plant growth and development, especially in economic crops. Although HAK/KUP/KT genes have been identified in many species, research on these genes in cotton is still quite rare. In this study, in total, 21, 24, 45, and 44 HAK/KUP/KT genes were identified in Gossypium arboreum, Gossypium raimondii, Gossypium hirsutum</ ...[more]

PMID: 33329704

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Project description:The HAK/KUP/KT (High-affinity K+ transporters/K+ uptake permeases/K+ transporters) is the largest and most dominant potassium transporter family in plants, playing a crucial role in various biological processes. However, our understanding of HAK/KUP/KT gene family in potato (Solanum tuberosum L.) remains limited and unclear. In this study, 24 HAK/KUP/KT genes (StHAKs) were identified through a genome-wide analysis and were found to be unevenly distributed across ten chromosomes. Based on phylogenetic analysis, these StHAK gene family members were classified into four distinct clusters. All StHAK protein sequences contained the conserved motifs and domains. Promoter cis-acting elements analysis revealed that most StHAK gene family members in potatoes were associated with responses to light and hormones such as abscisic acid or methyl jasmonate, however, many motifs responsive to hormones and stress conditions have not been clearly studied or reported in plants. Synteny analysis suggested that 33, 19, 8, 1 StHAK genes were orthologous to those in soybean, cassava, Arabidopsis and rice, respectively. The previously published RNA-seq results, transcriptomic data and qRT-PCR experiments indicated that the expression profiles of these StHAK genes were tissue-specific and were influenced by multiple factors, including biotic and abiotic stress, hormone, potassium fertilizer. To provide a clear and convenient view of StHAK gene expression across different tissues in potato, we generated a cartoon heatmap to vividly illustrate the tissue-specific expression of StHAK genes, which is unprecedented in the gene family analysis of potato. At last, we identified genes such as StHAK8, StHAK14, and StHAK22 with high expression in potato tubers using qRT-PCR, suggesting their potential involvement in tuber growth and development. This can contribute to a deeper understanding of the mechanism of potassium absorption and transportation in potatoes. It has laid a solid theoretical foundation for the genetic regulation of potassium nutritional efficiency in potatoes and the breeding of potato varieties with high potassium efficiency.

Dataset Information

Genome-Wide Identification and Expression Pattern Analysis of the HAK/KUP/KT Gene Family of Cotton in Fiber Development and Under Stresses.

Publications

Genome-Wide Identification and Expression Pattern Analysis of the <i>HAK/KUP/KT</i> Gene Family of Cotton in Fiber Development and Under Stresses.

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