Comparison of two ASC-derived therapeutics in an in vitro OA model: secretome versus extracellular vesicles.

ABSTRACT:

Background

In the last years, several clinical trials have proved the safety and efficacy of adipose-derived stem/stromal cells (ASC) in contrasting osteoarthritis (OA). Since ASC act mainly through paracrine mechanisms, their secretome (conditioned medium, CM) represents a promising therapeutic alternative. ASC-CM is a complex cocktail of proteins, nucleic acids, and lipids released as soluble factors and/or conveyed into extracellular vesicles (EV). Here, we investigate its therapeutic potential in an in vitro model of OA.

Methods

Human articular chondrocytes (CH) were induced towards an OA phenotype by 10 ng/ml TNFα in the presence of either ASC-CM or EV, both deriving from 5 × 10⁵ cells, to evaluate the effect on hypertrophic, catabolic, and inflammatory marke

SUBMITTER: Giannasi C 

PROVIDER: S-EPMC7711257 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature