Correlative light and electron microscopy analysis of the centrosome: A step-by-step protocol.

ABSTRACT: Correlative light and electron microscopy harnesses the best from each of the two modalities of microscopy it utilizes; while light microscopy provides information about the dynamic properties of the cellular structure or fluorescently labeled protein, electron microscopy provides ultrastructural information in an unsurpassed resolution. However, tracing a particular cell and its rare and small structures such as centrosomes throughout numerous steps of the experiment is not a trivial task. In this chapter, we present the experimental workflow for combining live-cell fluorescence microscopy analysis with classical transmission electron microscopy, adapted for the studies of the centrosomes and basal bodies. We describe, in a step-by-step manner, an approach that can be affordably and succe

SUBMITTER: Kong D 

PROVIDER: S-EPMC7711276 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature