Advanced Bioink for 3D Bioprinting of Complex Free-Standing Structures with High Stiffness.

ABSTRACT: One of the challenges in 3D-bioprinting is the realization of complex, volumetrically defined structures, that are also anatomically accurate and relevant. Towards this end, in this study we report the development and validation of a carboxylated agarose (CA)-based bioink that is amenable to 3D printing of free-standing structures with high stiffness at physiological temperature using microextrusion printing without the need for a fugitive phase or post-processing or support material (FRESH). By blending CA with negligible amounts of native agarose (NA) a bioink formulation (CANA) which is suitable for printing with nozzles of varying internal diameters under ideal pneumatic pressure was developed. The ability of the CANA ink to exhibit reproducible sol-gel transition at physiological temp

SUBMITTER: Gu Y 

PROVIDER: S-EPMC7711998 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature