Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering.

ABSTRACT:

Background

The physical factors of the extracellular matrix have a profound influence on the differentiation behavior of mesenchymal stem cells. In this study, the effect of the biophysical microenvironment on rat bone marrow mesenchymal stem cell (BMSC) osteogenesis was studied both in vitro and in vivo.

Methods

To prepare cell culture scaffolds of varying stiffness, increasing amounts of hydroxyapatite (HAp) were mixed into a polyethylene glycol/silk fibroin (PEG/SF) solution. The amount of HAp ranged from 25 to 100 mg, which provided for different ratios between HAp and the PEG/SF composite. In vitro, the effect of stiffness on the osteogenic differentiation of rat BMSCs was studied. The outcome measures, which were verified in vivo, included the protein expression of run

SUBMITTER: Yang Y 

PROVIDER: S-EPMC7712560 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature