Liquid biopsy for patients with IBD-associated neoplasia.

ABSTRACT:

Background

It is often difficult to diagnose inflammatory bowel disease (IBD)-associated neoplasia endoscopically due to background inflammation. In addition, due to the absence of sensitive tumor biomarkers, countermeasures against IBD-associated neoplasia are crucial. The purpose of this study is to develop a new diagnostic method through the application of liquid biopsy.

Methods

Ten patients with IBD-associated cancers and high-grade dysplasia (HGD) with preserved tumor tissue and blood were included. Tumor and non-tumor tissues were analyzed for 48 cancer-related genes using next-generation sequencing. Simultaneously, circulating tumor DNA (ctDNA) was analyzed for mutations in the target genes using digital PCR.

Results

Out of 10 patients, seven had IBD-related ca

SUBMITTER: Kinugasa H 

PROVIDER: S-EPMC7712625 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature