Dexamethasone and lidocaine suppress eosinophilopoiesis from umbilical cord blood cells.

ABSTRACT:

Background

Eosinophils play an important role in allergic inflammation. Glucocorticosteroids have been used as an anti-inflammatory medication for inflammatory diseases involving eosinophil infiltration. Some effect of nebulized lidocaine has been reported when treating certain patients with asthma, which is also an inflammatory disease. The goal of this study was to examine the effects of dexamethasone and lidocaine on eosinophil proliferation and differentiation using a model of human umbilical cord blood mononuclear cells (UCMC) cultured with IL-5.

Methods

UCMC were cultured with IL-5 (5 ng/mL) for 4 weeks. The effects of dexamethasone and lidocaine on the number and morphology of eosinophilic cells were visualized with Wright-Giemsa and cyanide-resistant peroxidase stain

SUBMITTER: Muraki M 

PROVIDER: S-EPMC7713138 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature