SKP1 promotes YAP-mediated colorectal cancer stemness via suppressing RASSF1.

ABSTRACT:

Background

Cancer stem cells (CSCs) have been recognized as an important drug target, however, the underlying mechanisms have not been fully understood. SKP1 is a traditional drug target for cancer therapy, while, whether SKP1 promotes colorectal cancer (CRC) stem cells (CRC-SCs) and the underlying mechanisms have remained elusive.

Methods

Human CRC cell lines and primary human CRC cells were used in this study. Gene manipulation was performed by lentivirus system. The mRNA and protein levels of target genes were examined by qRT-PCR and western blot. The sphere-forming and in vitro migration capacities were determined by sphere formation and transwell assay. The self-renewal was determined by limiting dilution assay. The tumorigenicity and metastasis of cancer cells were exa

SUBMITTER: Tian C 

PROVIDER: S-EPMC7713163 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature