Improve sample preparation process for miRNA isolation from the culture cells by using silica fiber membrane.

ABSTRACT: In clinical applications of miRNAs, the purity and quality of the testing samples are very critical, especially the obtained tissue sample volume is limited. If the extracted miRNAs are contaminated or different in quality before analysis, it will increase the variance of the analysis result and make the medical information judgment incorrect and cannot be portable. Herein, we improved the commercially extraction kit by realizing the fundamental mechanism and hoped to serve finding optimal procedures for increasing the recovery of miRNAs extracted from cultured cells. In the adsorption process, the factors, like increasing the ethanol concentration or adding Ca²⁺, could influence the RNA adsorption were investigated. For the elution process, the effect caused by raising the elut

SUBMITTER: Hu WP 

PROVIDER: S-EPMC7713297 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature