Role of aspartate ammonia-lyase in Pasteurella multocida.

ABSTRACT:

Background

Pasteurella multocida is responsible for a highly infectious and contagious disease in birds, leading to heavy economic losses in the chicken industry. However, the pathogenesis of this disease is poorly understood. We recently identified an aspartate ammonia-lyase (aspA) in P. multocida that was significantly upregulated under iron-restricted conditions, the protein of which could effectively protect chicken flocks against P. multocida. However, the functions of this gene remain unclear. In the present study, we constructed aspA mutant strain △aspA::kan and complementary strain C△aspA::kan to investigate the function of aspA in detail.

Result

Deletion of the aspA gene in P. multocida resulted in a significant reduction in bacterial growth in LB (Luria-Bertani) an

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7713322 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature