Lipopolysaccharide exposure induces oxidative damage in Caenorhabditis elegans: protective effects of carnosine.

ABSTRACT:

Background

The present study was designed to investigate the protective effects and mechanisms of carnosine on lipopolysaccharide (LPS)-induced injury in Caenorhabditis elegans.

Methods

C. elegans individuals were stimulated for 24 h with LPS (100 μg/mL), with or without carnosine (0.1, 1, 10 mM). The survival rates and behaviors were determined. The activities of superoxide dismutase (SOD), glutathione reductase (GR), and catalase (CAT) and levels of malondialdehyde (MDA) and glutathione (GSH) were determined using the respective kits. Reverse transcription polymerase chain reaction (RT-PCR) was performed to validate the differential expression of sod-1, sod-2, sod-3, daf-16, ced-3, ced-9, sek-1, and pmk-1. Western blotting was used to determine the levels of SEK1, p38 mito

SUBMITTER: Ma J 

PROVIDER: S-EPMC7713333 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature