Identification of novel alternative splicing biomarkers for breast cancer with LC/MS/MS and RNA-Seq.

ABSTRACT:

Background

Alternative splicing isoforms have been reported as a new and robust class of diagnostic biomarkers. Over 95% of human genes are estimated to be alternatively spliced as a powerful means of producing functionally diverse proteins from a single gene. The emergence of next-generation sequencing technologies, especially RNA-seq, provides novel insights into large-scale detection and analysis of alternative splicing at the transcriptional level. Advances in Proteomic Technologies such as liquid chromatography coupled tandem mass spectrometry (LC-MS/MS), have shown tremendous power for the parallel characterization of large amount of proteins in biological samples. Although poor correspondence has been generally found from previous qualitative comparative analysis between pro

SUBMITTER: Zhang F 

PROVIDER: S-EPMC7713335 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature