Common and personal target genes of the micronutrient vitamin D in primary immune cells from human peripheral blood.

ABSTRACT: Vitamin D is essential for the function of the immune system. In this study, we treated peripheral blood mononuclear cells (PBMCs) of healthy adults with the biologically active form of vitamin D₃, 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) using two different approaches: single repeats with PBMCs obtained from a cohort of 12 individuals and personalized analysis based on triplicates of five study participants. This identified 877 (cohort approach) and 3951 (personalized approach) genes that significantly (p < 0.05) changed their expression 24 h after 1,25(OH)₂D₃ stimulation. From these, 333 and 1232 were classified as supertargets, a third of which were identified as novel. Individuals differed largely in their vitamin D respo

SUBMITTER: Hanel A 

PROVIDER: S-EPMC7713372 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature