Using high-resolution melting to identify Calliphoridae (blowflies) species from Brazil.

ABSTRACT: Forensic entomology is the study of insects and other arthropods used in the solution of crimes. Most of entomological evidences strongly depend on accurate species identification. Therefore, new methods are being developed due to difficulties in morphological identification, including molecular methods such as High-Resolution Melting. In this study, we reported a new HRM primer set to identify forensically important Calliphoridae (blowflies) from Brazil. For such purpose, Calliphoridae species of forensic importance in Brazil were listed and confirmed by specialists. Mitochondrial COI sequences of those species were downloaded from databases and aligned, and polymorphic variations were selected for distinction between species. Based on it, HRM primers were designed. Forty-three fly sample

SUBMITTER: Oliveira PV 

PROVIDER: S-EPMC7713596 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature