Effects of platelet-rich plasma on mesenchymal stem cells isolated from rat uterus.

ABSTRACT:

Background

Platelet-rich plasma (PRP), which represents a valuable source of growth factors, is increasingly being applied in regenerative medicine. Recent findings suggest the feasibility of using PRP in the treatment of infertility secondary to refractory thin endometrium. Mesenchymal stem/stromal cells (MSCs) of the endometrium are an essential cellular component responsible for extracellular matrix remodeling, angiogenesis, cell-to-cell communication, and postmenstrual tissue repair. Using a rat model, we examine the effects of autologous PRP on MSCs isolated from the uterus and compare them with the effects of autologous ordinary plasma (OP) and complete growth medium.

Methods

MSCs were isolated from uterine tissues via enzymatic disaggregation. Flow cytometry immunophe

SUBMITTER: Vishnyakova P 

PROVIDER: S-EPMC7713597 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature