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Feasibility of collection and analysis of microbiome data in a longitudinal randomized trial of community gardening.


ABSTRACT: Aim: We explored the feasibility of collecting and analyzing human microbiome data in a longitudinal randomized controlled trial of community gardening. Methods & materials: Participants were randomly assigned to gardening (N = 8) or control (N = 8). Participants provided stool, mouth, hand and forehead microbiome samples at six timepoints. Analyses combined mixed models with Qiita output. Results: Participant satisfaction was high, with 75% of participants completing evaluations. While no microbial effects were statistically significant due to small sample size, the analysis pipeline utility was tested. Conclusion: Longitudinal collection and analysis of microbiome data in a community gardening randomized controlled trial is feasible. The analysis pipeline will be useful in larger studies for assessment of the pathway between microbiota, gardening and health outcomes.

SUBMITTER: Gascon M 

PROVIDER: S-EPMC7713898 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Feasibility of collection and analysis of microbiome data in a longitudinal randomized trial of community gardening.

Gascon Mireia M   Harrall Kylie K KK   Beavers Alyssa W AW   Glueck Deborah H DH   Stanislawski Maggie A MA   Alaimo Katherine K   Villalobos Angel A   Hebert James R JR   Dexter Kelsey K   Li Kaigang K   Litt Jill J  

Future microbiology 20200501


<b>Aim:</b> We explored the feasibility of collecting and analyzing human microbiome data in a longitudinal randomized controlled trial of community gardening. <b>Methods & materials:</b> Participants were randomly assigned to gardening (N = 8) or control (N = 8). Participants provided stool, mouth, hand and forehead microbiome samples at six timepoints. Analyses combined mixed models with Qiita output. <b>Results:</b> Participant satisfaction was high, with 75% of participants completing evalua  ...[more]

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