A priori estimation of sequencing effort in complex microbial metatranscriptomes.

ABSTRACT: Metatranscriptome analysis or the analysis of the expression profiles of whole microbial communities has the additional challenge of dealing with a complex system with dozens of different organisms expressing genes simultaneously. An underlying issue for virtually all metatranscriptomic sequencing experiments is how to allocate the limited sequencing budget while guaranteeing that the libraries have sufficient depth to cover the breadth of expression of the community. Estimating the required sequencing depth to effectively sample the target metatranscriptome using RNA-seq is an essential first step to obtain robust results in subsequent analysis and to avoid overexpansion, once the information contained in the library reaches saturation. Here, we present a method to calculate the sequencin

SUBMITTER: Monleon-Getino T 

PROVIDER: S-EPMC7713979 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature