Activation of the receptor tyrosine kinase RET improves long-term hematopoietic stem cell outgrowth and potency.

ABSTRACT: Expansion of human hematopoietic stem cells (HSCs) is a rapidly advancing field showing great promise for clinical applications. Recent evidence has implicated the nervous system and glial family ligands (GFLs) as potential drivers of hematopoietic survival and self-renewal in the bone marrow niche; how to apply this process to HSC maintenance and expansion has yet to be explored. We show a role for the GFL receptor, RET, at the cell surface of HSCs in mediating sustained cellular growth, resistance to stress, and improved cell survival throughout in vitro expansion. HSCs treated with the key RET ligand/coreceptor complex, glial-derived neurotrophic factor and its coreceptor, exhibit improved progenitor function at primary transplantation and improved long-term HSC function at secondary tr

SUBMITTER: Grey W 

PROVIDER: S-EPMC7714096 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature