Intravital imaging reveals cell cycle-dependent myogenic cell migration during muscle regeneration.

ABSTRACT: During muscle regeneration, extracellular signal-regulated kinase (ERK) promotes both proliferation and migration. However, the relationship between proliferation and migration is poorly understood in this context. To elucidate this complex relationship on a physiological level, we established an intravital imaging system for measuring ERK activity, migration speed, and cell-cycle phases in mouse muscle satellite cell-derived myogenic cells. We found that in vivo, ERK is maximally activated in myogenic cells two days after injury, and this is then followed by increases in cell number and motility. With limited effects of ERK activity on migration on an acute timescale, we hypothesized that ERK increases migration speed in the later phase by promoting cell-cycle progression. Our cell

SUBMITTER: Konagaya Y 

PROVIDER: S-EPMC7714504 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature