Imaging Single Bacterial Cells with Electro-optical Impedance Microscopy.

ABSTRACT: Impedance measurements have been an important tool for biosensor applications, including protein detection, DNA quantification, and cell study. We present here an electro-optical impedance microscopy (EIM) based on the dependence of surface optical transmission on local surface charge density for single bacteria impedance imaging. We applied a potential modulation to bacteria placed on an indium tin oxide-coated slide and simultaneously recorded a sequence of transmitted microscopy images. By performing fast Fourier transform analysis on the image sequences, we obtained the DC component (signal at 0 Hz) for cell morphology imaging and the AC component (signal at the modulation frequency) for the mapping of cell impedance responses with subcellular resolution for the first time. Using this

SUBMITTER: Zhang F 

PROVIDER: S-EPMC7714712 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature