Comparison of Transparency and Shrinkage During Clearing of Insect Brains Using Media With Tunable Refractive Index.

ABSTRACT: Improvement of imaging quality has the potential to visualize previously unseen building blocks of the brain and is therefore one of the great challenges in neuroscience. Rapid development of new tissue clearing techniques in recent years have attempted to solve imaging compromises in thick brain samples, particularly for high resolution optical microscopy, where the clearing medium needs to match the high refractive index of the objective immersion medium. These problems are exacerbated in insect tissue, where numerous (initially air-filled) tracheal tubes branching throughout the brain increase the scattering of light. To date, surprisingly few studies have systematically quantified the benefits of such clearing methods using objective transparency and tissue shrinkage measurements. In t

SUBMITTER: Bekkouche BMB 

PROVIDER: S-EPMC7714936 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature