Comparative growth potential of thermophilic amylolytic Bacillus sp. on unconventional media food wastes and its industrial application.

ABSTRACT: Amylases take part with vital role in industries such as food, fermentation; starch processing, textile and paper etc. Increasing amylases demand, high nutrient expenditure and environmental pollution have forced to utilize agro-industrial residues as a low-cost feedstock for enzyme production. In present study, three soil samples were collected from agro-industrial waste dumping areas in District Faisalabad. Ten thermophilic bacterial isolates were separated at 55 °C on the basis of colonial morphology, three isolates (F6, F11, F17) showed prominent zone of clearance applying iodine test on starch agar plates. Bacterial isolate F-11 showed highest amylase activity with DNS method and molecularly identified through 16S RNA sequencing as Bacillus sp. with Accession number MH917294. F

SUBMITTER: Saleh F 

PROVIDER: S-EPMC7715428 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature