Dataset Information

Mycoplasma bovis in Nordic European Countries: Emergence and Dominance of a New Clone.

ABSTRACT: Mycoplasma (M.) bovis is an important pathogen of cattle implicated in a broad range of clinical manifestations that adversely impacts livestock production worldwide. In the absence of a safe, effective commercial vaccine in Europe, the reported reduced susceptibility to antimicrobials for this organism has contributed to difficulties in controlling infection. Despite global presence, some countries have only recently experienced outbreaks of this pathogen. In the present study, M. bovis isolates collected in Denmark between 1981 and 2016 were characterized to determine (i) genetic diversity and phylogenetic relationships using whole genome sequencing and various sequence-based typing methods and (ii) patterns of antimicrobial resistance compared to other European isolates. The M. bovis population in Denmark was found to be highly homogeneous genomically and with respect to the antimicrobial resistance profile. Previously dominated by an old genotype shared by many other countries (ST17 in the PubMLST legacy scheme), a new predominant type represented by ST94-adh1 has emerged. The same clone is also found in Sweden and Finland, where M. bovis introduction is more recent. Although retrieved from the Netherlands, it appears absent from France, two countries with a long history of M. bovis infection where the M. bovis population is more diverse.

SUBMITTER: Tardy F

PROVIDER: S-EPMC7716209 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Mycoplasma bovis in Nordic European Countries: Emergence and Dominance of a New Clone.

Tardy Florence F Aspan Anna A Autio Tiina T Ridley Anne A Tricot Agnès A Colin Adélie A Pohjanvirta Tarja T Smid Bregtje B Harders Frank F Lindegaard Mikkel M Tølbøll Lauritsen Klara K Lyhs Ulrike U Wisselink Henk J HJ Strube Mikael Lenz ML

Pathogens (Basel, Switzerland) 20201023 11

Mycoplasma (M.) bovis is an important pathogen of cattle implicated in a broad range of clinical manifestations that adversely impacts livestock production worldwide. In the absence of a safe, effective commercial vaccine in Europe, the reported reduced susceptibility to antimicrobials for this organism has contributed to difficulties in controlling infection. Despite global presence, some countries have only recently experienced outbreaks of this pathogen. In the present st ...[more]

PMID: 33114269

Similar Datasets

Project description:BackgroundSeveral species-specific PCR assays, based on a variety of target genes are currently used in the diagnosis of Mycoplasma bovis infections in cattle herds with respiratory diseases and/or mastitis. With this diversity of methods, and the development of new methods and formats, regular performance comparisons are required to ascertain diagnostic quality. The present study compares PCR methods that are currently used in six national veterinary institutes across Europe. Three different sample panels were compiled and analysed to assess the analytical specificity, analytical sensitivity and comparability of the different PCR methods. The results were also compared, when appropriate, to those obtained through isolation by culture. The sensitivity and comparability panels were composed of samples from bronchoalveolar fluids of veal calves, artificially contaminated or naturally infected, and hence the comparison of the different methods included the whole workflow from DNA extraction to PCR analysis.ResultsThe participating laboratories used i) five different DNA extraction methods, ii) seven different real-time and/or end-point PCRs targeting four different genes and iii) six different real-time PCR platforms. Only one commercial kit was assessed; all other PCR assays were in-house tests adapted from published methods. The analytical specificity of the different PCR methods was comparable except for one laboratory where Mycoplasma agalactiae was tested positive. Frequently, weak-positive results with Ct values between 37 and 40 were obtained for non-target Mycoplasma strains. The limit of detection (LOD) varied from 10 to 103 CFU/ml to 103 and 106 CFU/ml for the real-time and end-point assays, respectively. Cultures were also shown to detect concentrations down to 102 CFU/ml. Although Ct values showed considerable variation with naturally infected samples, both between laboratories and tests, the final result interpretation of the samples (positive versus negative) was essentially the same between the different laboratories.ConclusionWith a few exceptions, all methods used routinely in the participating laboratories showed comparable performance, which assures the quality of diagnosis, despite the multiplicity of the methods.

Project description:Livestock production is a fundamental pillar of the Moroccan economy. Infectious diseases of cattle and other species represent a significant threat to the livestock industry, animal health, and food safety. Bovine tuberculosis (bTB), mainly caused by Mycobacterium bovis, generates considerable direct and indirect economic losses, and an underestimated human health burden caused by zoonotic transmission. Previous studies have suggested likely M. bovis transmission links between Morocco and Southern Europe, however, limitations inherent with the methods used prevented definitive conclusions. In this study, we employed whole genome sequencing analysis to determine the genetic diversity of the first 55 M. bovis whole-genomes in Morocco and to better define the phylogenetic links between strains from Morocco and a large dataset from related and neighboring countries. With a total of 780 M. bovis sequences extracted from cattle, wildlife or humans and representing 36 countries, we discovered two new M bovis spoligotypes in Morocco and that the Moroccan clonal complexes are classified as belonging to Europe or Unknown, supporting previous studies that the Sahara Desert might be playing a key role in preventing M. bovis transmission between North Africa and Sub-Saharan Africa. Furthermore, our analysis showed a close M. bovis genetic relationship between cattle from Morocco and cattle from Spain, France, Portugal and Germany, and from cattle in Morocco and humans in Italy, Germany, and the UK. These results suggest that animal trade and human migration between Morocco and these countries might be playing a role in disease transmission. Our study benefits from a large sample size and a rich dataset that includes sequences from cattle, wildlife and humans from Morocco and neighboring countries, enabling the delineation of M. bovis genetic links across countries and host-species. Our study calls for further investigation of animal and zoonotic TB spread in Morocco and in other countries, which is important to inform future TB control measures at the animal-human interface.

Dataset Information

Mycoplasma bovis in Nordic European Countries: Emergence and Dominance of a New Clone.

Publications

<i>Mycoplasma bovis</i> in Nordic European Countries: Emergence and Dominance of a New Clone.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets