A rapid, cost-effective tailed amplicon method for sequencing SARS-CoV-2.

ABSTRACT:

Background

The global COVID-19 pandemic has led to an urgent need for scalable methods for clinical diagnostics and viral tracking. Next generation sequencing technologies have enabled large-scale genomic surveillance of SARS-CoV-2 as thousands of isolates are being sequenced around the world and deposited in public data repositories. A number of methods using both short- and long-read technologies are currently being applied for SARS-CoV-2 sequencing, including amplicon approaches, metagenomic methods, and sequence capture or enrichment methods. Given the small genome size, the ability to sequence SARS-CoV-2 at scale is limited by the cost and labor associated with making sequencing libraries.

Results

Here we describe a low-cost, streamlined, all amplicon-based method for s

SUBMITTER: Gohl DM 

PROVIDER: S-EPMC7716288 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature