PerSort Facilitates Characterization and Elimination of Persister Subpopulation in Mycobacteria.

ABSTRACT: Mycobacterium tuberculosis (MTB) generates phenotypic diversity to persist and survive the harsh conditions encountered during infection. MTB avoids immune effectors and antibacterial killing by entering into distinct physiological states. The surviving cells, persisters, are a major barrier to the timely and relapse-free treatment of tuberculosis (TB). We present for the first time, PerSort, a method to isolate and characterize persisters in the absence of antibiotic or other pressure. We demonstrate the value of PerSort to isolate translationally dormant cells that preexisted in small numbers within Mycobacterium species cultures growing under optimal conditions but that dramatically increased in proportion under stress conditions. The translationally dormant subpopulation

SUBMITTER: Srinivas V 

PROVIDER: S-EPMC7716392 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature