Long noncoding RNA NEAT1 regulates radio-sensitivity via microRNA-27b-3p in gastric cancer.

ABSTRACT:

Background

Long noncoding RNA nuclear-enriched abundant transcript 1 (NEAT1) exhibits an oncogenic role in multiple cancers, including gastric cancer (GC). But, the functions of NEAT1 in modulating radio-sensitivity of GC and its potential molecular mechanisms have not been totally elucidated.

Methods

qRT-PCR was performed to detect the expressions of NEAT1 and microRNA-27b-3p (miR-27b-3p). Kaplan-Meier survival curves for NEAT1 expression in GC created using KM Plotter. Colony formation assay was used to determine the survival fraction. Cell apoptosis was evaluated by flow cytometry. Luciferase reporter assay was used to verify the relationship between miR-27b-3p and NEAT1.

Results

NEAT1 was highly expressed while miR-27b-3p was downregulated in GC tissues and cells.

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC7716475 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature