The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.

ABSTRACT:

Purpose

Growing evidence indicates that aberrant expression of microRNAs contributes to tumor development. However, the biological role of microRNA-4490 (miR-4490) in gastric cancer (GC) remains to be clarified.

Methods

To explore the function of miR-4490 in GC, we performed colony formation, EdU incorporation, qRT-PCR, Western blotting, in situ hybridization (ISH), immunohistochemistry (IHC), flow cytometry, ChIP and dual-luciferase reporter assays. In addition, the growth, migration and invasion capacities of GC cells were evaluated.

Results

We found that miR-4490 was significantly downregulated in primary GC samples and in GC-derived cell lines compared with normal controls, and that this expression level was negatively correlated with GC malignancy. Exogenous miR-

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC7716863 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature