RamA, a transcriptional regulator conferring florfenicol resistance in Leclercia adecarboxylata R25.

ABSTRACT: Due to the inappropriate use of florfenicol in agricultural practice, florfenicol resistance has become increasingly serious. In this work, we studied the novel florfenicol resistance mechanism of an animal-derived Leclercia adecarboxylata strain R25 with high-level florfenicol resistance. A random genomic DNA library was constructed to screen the novel florfenicol resistance gene. Gene cloning, gene knockout, and complementation combined with the minimum inhibitory concentration (MIC) detection were conducted to determine the function of the resistance-related gene. Sequencing and bioinformatics methods were applied to analyze the structure of the resistance gene-related sequences. Finally, we obtained a regulatory gene of an RND (resistance-nodulation-cell division) system, ramA, that co

SUBMITTER: Cheng C 

PROVIDER: S-EPMC7716942 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature