Evaluation of LAMP for detection of Shigella from stool samples in children.

ABSTRACT:

Background

To assess the diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for the detection of Shigella from stool samples from children.

Methods

Consecutive stool samples from children aged <13 years old who presented with acute watery diarrhoea or dysentery to the Department of Paediatrics were collected and processed in the Department of Microbiology. All the stool samples were subjected to culture, conventional PCR and LAMP. Genomic sequencing was performed for samples that were positive by LAMP but negative by both culture and conventional PCR. The LAMP results were compared to those from culture and to a composite reference standard based on culture and conventional PCR.

Results

Amongst the 374 stool samples tested, 291 samples were po

SUBMITTER: Raghavan R 

PROVIDER: S-EPMC7717480 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature