A novel ultrasound-guided mouse model of sudden cardiac arrest.

ABSTRACT:

Aim

Mouse models of sudden cardiac arrest are limited by challenges with surgical technique and obtaining reliable venous access. To overcome this limitation, we sought to develop a simplified method in the mouse that uses ultrasound-guided injection of potassium chloride directly into the heart.

Methods

Potassium chloride was delivered directly into the left ventricular cavity under ultrasound guidance in intubated mice, resulting in immediate asystole. Mice were resuscitated with injection of epinephrine and manual chest compressions and evaluated for survival, body temperature, cardiac function, kidney damage, and diffuse tissue injury.

Results

The direct injection sudden cardiac arrest model causes rapid asystole with high surgical survival rates and short surgica

SUBMITTER: Rutledge CA 

PROVIDER: S-EPMC7717537 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature