A novel class of polymeric fluorescent dyes assembled using a DNA synthesizer.

ABSTRACT: In the pursuit of a novel class of fluorescent dyes we have developed a programmable polymer system that enables the rational design and control of macromolecular constructs through simple control of polymer primary sequence. These polymers are assembled using standard phosphoramidite chemistry on a DNA synthesizer which allows for extremely rapid prototyping and enables many permutations due to the large selection of phosphoramidite monomers presently available on the market. This programmability to some extent allows us to control the interactions/spacing of payload molecules distributed along the designed polymeric backbone. Control of molecular architecture using this technology has allowed us to address the long-standing technical issue of contact quenching between fluorescent dyes of

SUBMITTER: Matray T 

PROVIDER: S-EPMC7717558 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature