Detection of Crimean Congo haemorrhagic fever virus in North-eastern Senegal, Bokidiawe 2019.
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SUBMITTER: Dieng I
PROVIDER: S-EPMC7717587 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
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Detection of Crimean Congo haemorrhagic fever virus in North-eastern Senegal, Bokidiawé 2019.
Dieng Idrissa I Barry Mamadou Aliou MA Diagne Moussa Moise MM Diop Boly B Ndiaye Mamadou M Faye Martin M Ndione Marie Henriette Dior MHD Dieng Mame Malick MM Bousso Abdoulaye A Fall Gamou G Loucoubar Cheikh C Sall Amadou Alpha AA Faye Oumar O Faye Ousmane O
Emerging microbes & infections 20201201 1
We diagnosed a human case of Crimean Congo hemorrhagic fever (CCHF) in Bokidiawe (North-eastern Senegal), 2019. The phylogenetic analysis revealed that the isolate belongs to genotype III and is closely related to a strain reported in Mauritania in 1984 and Spain in 2016. Distribution area of CCHF in Senegal is progressively increasing. ...[more]
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Project description:Crimean-Congo hemorrhagic fever (CCHF), the most widespread tick-borne viral human infection, poses a threat to global health. In this study, clinical samples collected through national surveillance systems were screened for acute CCHF virus (CCHFV) infection using RT-PCR and for exposure using ELISA. For any CCHF-positive sample, livestock and tick samples were also collected in the neighborhood of the confirmed case and tested using ELISA and RT-PCR, respectively. Genome sequencing and phylogenetic analyses were also performed on samples with positive RT-PCR results. In Eastern Senegal, two human cases and one Hyalomma tick positive for CCHF were identified and a seroprevalence in livestock ranging from 9.33% to 45.26% was detected. Phylogenetic analyses revealed that the human strain belonged to genotype I based on the available L segment. However, the tick strain showed a reassortant profile, with the L and M segments belonging to genotype I and the S segment belonging to genotype III. Our data also showed that our strains clustered with strains isolated in different countries, including Mauritania. Therefore, our findings confirmed the high genetic variability inside the CCHF genotypes and their introduction to Senegal from other countries. They also indicate an increasing CCHF threat in Senegal and emphasize the need to reinforce surveillance using a one-health approach.
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