Assessment of hepatic pyruvate carboxylase activity using hyperpolarized [1-¹³ C]-l-lactate.

ABSTRACT:

Purpose

To evaluate the utility of hyperpolarized [1-¹³ C]-l-lactate to detect hepatic pyruvate carboxylase activity in vivo under fed and fasted conditions.

Methods

[1-¹³ C]-labeled sodium L-lactate was polarized using a dynamic nuclear polarizer. Polarization level and the T₁ were measured in vitro in a 3 Telsa MR scanner. Two groups of healthy rats (fasted vs. fed) were prepared for in vivo studies. Each rat was anesthetized and intravenously injected with 60-mM hyperpolarized [1-¹³ C]-l-lactate, immediately followed by dynamic acquisition of ¹³ C (carbon-13) MR spectra from the liver at 3 Tesla. The dosage-dependence of the ¹³ C-products was also investigated by performing another injection of an equal vol

SUBMITTER: Chen J 

PROVIDER: S-EPMC7718288 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature