Novel method to quantify phenotypic markers of HIV-associated neurocognitive disorder in a murine SCID model.

ABSTRACT: Despite combined antiretroviral therapy (cART), HIV infection in the CNS persists with reported increases in activation of macrophages (MΦ), microglia, and surrounding astrocytes/neurons, conferring HIV-induced inflammation. Chronic inflammation results in HIV-associated neurocognitive disorders (HAND) with reported occurrence of up to half of individuals with HIV infection. The existing HAND mouse model used by laboratories including ours, and the effect of novel agents on its pathology present with labor-intensive and time-consuming limitations since brain sections and immunohistochemistry assays have to be performed and analyzed. A novel flow cytometry-based system to objectively quantify phenotypic effects of HIV using a SCID mouse HAND model was developed which demonstrated that the H

SUBMITTER: Gavegnano C 

PROVIDER: S-EPMC7718289 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature