Subcellular localisation and composition of intramuscular triacylglycerol influence insulin sensitivity in humans.

ABSTRACT:

Aims/hypothesis

Subcellular localisation is an important factor in the known impact of bioactive lipids, such as diacylglycerol and sphingolipids, on insulin sensitivity in skeletal muscle; yet, the role of localised intramuscular triacylglycerol (IMTG) is yet to be described. Excess accumulation of IMTG in skeletal muscle is associated with insulin resistance, and we hypothesised that differences in subcellular localisation and composition of IMTG would relate to metabolic health status in humans.

Methods

We evaluated subcellular localisation of IMTG in lean participants, endurance-trained athletes, individuals with obesity and individuals with type 2 diabetes using LC-MS/MS of fractionated muscle biopsies and insulin clamps.

Results

Insulin sensitivity was significa

SUBMITTER: Kahn D 

PROVIDER: S-EPMC7718332 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature