Force dependent effects of chronic overuse on fibrosis-related genes and proteins in skeletal muscles.

ABSTRACT:

Aim

To examine the chronic effect of force on mRNA and protein expression levels of fibrosis-related genes in flexor digitorum muscles in a rat model of repetitive overuse injury that induces muscle fibrosis at high force levels.

Materials and methods

Two groups of rats were trained to perform a voluntary repetitive lever-pulling task at either a high (HFHR) or a low force (LFHR) for 18 weeks, while a control group (FRC) performed no task. RNA and protein were prepared from forelimb flexor digitorum muscles. Fibrosis-related gene RNA transcripts were evaluated using quantitative PCR (qPCR) and analyzed using the geometric mean of three housekeeping genes or the mean of each individually as reference. Protein levels were quantified using ELISA, western blot, or immunohistoflu

SUBMITTER: Hilliard BA 

PROVIDER: S-EPMC7718395 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature