Mechanism and quantitative assessment of saturation transfer for water-based detection of the aliphatic protons in carbohydrate polymers.

ABSTRACT:

Purpose

CEST MRI experiments of mobile macromolecules, for example, proteins, carbohydrates, and phospholipids, often show signals due to saturation transfer from aliphatic protons to water. Currently, the mechanism of this nuclear Overhauser effect (NOE)-based transfer pathway is not completely understood and could be due either to NOEs directly to bound water or NOEs relayed intramolecularly via exchangeable protons. We used glycogen as a model system to investigate this saturation transfer pathway in sugar polymer solution.

Methods

To determine whether proton exchange affected saturation transfer, saturation spectra (Z-spectra) were measured for glycogen solutions of different pH, D₂ O/H₂ O ratio, and glycogen particle size. A theoretical model was d

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC7718412 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature