Oxidative Stress Responses and Nutrient Starvation in MCHM Treated Saccharomyces cerevisiae.

ABSTRACT: In 2014, the coal cleaning chemical 4-methylcyclohexane methanol (MCHM) spilled into the water supply for 300,000 West Virginians. Initial toxicology tests showed relatively mild results, but the underlying effects on cellular biology were underexplored. Treated wildtype yeast cells grew poorly, but there was only a small decrease in cell viability. Cell cycle analysis revealed an absence of cells in S phase within thirty minutes of treatment. Cells accumulated in G1 over a six-hour time course, indicating arrest instead of death. A genetic screen of the haploid knockout collection revealed 329 high confidence genes required for optimal growth in MCHM. These genes encode three major cell processes: mitochondrial gene expression/translation, the vacuolar ATPase, and aromatic amino acid bios

SUBMITTER: Ayers MC 

PROVIDER: S-EPMC7718757 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature