Slow Growth and Increased Spontaneous Mutation Frequency in Respiratory Deficient afo1 ^- Yeast Suppressed by a Dominant Mutation in ATP3.

ABSTRACT: A yeast deletion mutation in the nuclear-encoded gene, AFO1, which codes for a mitochondrial ribosomal protein, led to slow growth on glucose, the inability to grow on glycerol or ethanol, and loss of mitochondrial DNA and respiration. We noticed that afo1 ^- yeast readily obtains secondary mutations that suppress aspects of this phenotype, including its growth defect. We characterized and identified a dominant missense suppressor mutation in the ATP3 gene. Comparing isogenic slowly growing rho-zero and rapidly growing suppressed afo1 ^- strains under carefully controlled fermentation conditions showed that energy charge was not significantly different between strains and was not causal for the observed growth properties. Surprisingly, in a w

SUBMITTER: Li J 

PROVIDER: S-EPMC7718765 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature