Mitochondria as a Platform for Dictating the Cell Fate of Cultured Human Corneal Endothelial Cells.

ABSTRACT:

Purpose

Aiming to clarify the role of mitochondria in cell fate decision of cultured human corneal endothelial cell (cHCEC) subpopulations.

Methods

The mitochondrial respiratory ability were examined with Mito stress and Mito fuel flex test assays using an extracellular flux analyzer (XFe24; Agilent Technologies; Santa Clara, CA) for human corneal endothelium tissues, mature cHCECs and a variety of cell state transitioned cHCECs. Tricarboxylic acid cycle and acetyl-coenzyme A-related enzymes was analyzed by proteomics for cell lysates using liquid chromatography-tandem mass spectrometry for cHCEC subpopulations.

Results

The maximum oxygen consumption rate was found to become stable depending on the maturation of cHCECs. In the Mito stress tests, culture supplements, e

SUBMITTER: Numa K 

PROVIDER: S-EPMC7718813 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature