Alpha-Adrenergic Agonists Stimulate Fluid Secretion in Lacrimal Gland Ducts.

ABSTRACT:

Purpose

The role of adrenergic innervation in the regulation of lacrimal gland (LG) ductal fluid secretion is unknown. The Aim of the present study was to investigate the effect of adrenergic stimulation on fluid secretion in isolated LG duct segments and to study the underlying intracellular mechanisms.

Methods

Fluid secretion of isolated mouse LG ducts was measured using video-microscopy. Effect of various adrenergic agonists (norepinephrine, phenylephrine, and isoproterenol) on fluid secretion as well as inhibitory effects of specific antagonists on adrenergic agonist-stimulated secretory response were analyzed. Changes in intracellular Ca2+ level [Ca2+i] were investigated with microfluorometry.

Results

Both norepinephrine and phenylephrine initiated a rapid and ro

SUBMITTER: Szarka D 

PROVIDER: S-EPMC7718821 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature