Identification and characterization of antigen-specific CD4⁺ T cells targeting renally expressed antigens in human lupus nephritis with two independent methods.

ABSTRACT: In the search for anti-renal autoreactivity in human lupus nephritis, we stimulated blood-derived CD4⁺ T cells from patients with systemic lupus erythematosus with various kidney lysates. Although only minor responses were detectable, these experiments led to the development of a search algorithm that combined autoantibody association with human lupus nephritis and target gene expression in inflamed kidneys. Applying this algorithm, five potential T cell antigens were identified. Blood-derived CD4⁺ T cells were then stimulated with these antigens. The cells were magnetically enriched prior to measurement with flow cytometry to facilitate the detection of very rare autoantigen-specific cells. The detected responses were dominated by IFN-γ-producing CD4⁺ T ce

SUBMITTER: Tesch S 

PROVIDER: S-EPMC7718878 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature