The role of menopause in tenofovir diphosphate and emtricitabine triphosphate concentrations in cervical tissue.

ABSTRACT:

Objective

Although postmenopausal (post-M) women have behavioral and biological risk factors for HIV infection, the activity of preexposure prophylaxis (PrEP) agents in older adults has not been well studied.

Design

We used an ex-vivo approach to compare the tissue concentrations of tenofovir (TFV) diphosphate (TFVdp) and emtricitabine (FTC) triphosphate (FTCtp) in cervical tissues from premenopausal (pre-M) and post-M women.

Method

Cervical explants from 16 pre-M and 11 post-M women were incubated in 10-300 μg/ml TFV or FTC for 24 h. Explants were then snap frozen in liquid nitrogen and stored until analysis. TFVdp and FTCtp were quantified using tandem liquid chromatography-mass spectrometry.

Results

Active metabolite concentrations of TFVdp were more than ni

SUBMITTER: Nicol MR 

PROVIDER: S-EPMC7719012 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature