Rapid bioprinting of conjunctival stem cell micro-constructs for subconjunctival ocular injection.

ABSTRACT: Ocular surface diseases including conjunctival disorders are multifactorial progressive conditions that can severely affect vision and quality of life. In recent years, stem cell therapies based on conjunctival stem cells (CjSCs) have become a potential solution for treating ocular surface diseases. However, neither an efficient culture of CjSCs nor the development of a minimally invasive ocular surface CjSC transplantation therapy has been reported. Here, we developed a robust in vitro expansion method for primary rabbit-derived CjSCs and applied digital light processing (DLP)-based bioprinting to produce CjSC-loaded hydrogel micro-constructs for injectable delivery. Expansion medium containing small molecule cocktail generated fast dividing and highly homogenous CjSCs for more than 10 pa

SUBMITTER: Zhong Z 

PROVIDER: S-EPMC7719077 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature