Myosin dynamics during relaxation in mouse soleus muscle and modulation by 2'-deoxy-ATP.

ABSTRACT:

Key points

Skeletal muscle relaxation has been primarily studied by assessing the kinetics of force decay. Little is known about the resultant dynamics of structural changes in myosin heads during relaxation. The naturally occurring nucleotide 2-deoxy-ATP (dATP) is a myosin activator that enhances cross-bridge binding and kinetics. X-ray diffraction data indicate that with elevated dATP, myosin heads were extended closer to actin in relaxed muscle and myosin heads return to an ordered, resting state after contraction more quickly. Molecular dynamics simulations of post-powerstroke myosin suggest that dATP induces structural changes in myosin heads that increase the surface area of the actin-binding regions promoting myosin interaction with actin, which could explain the observed de

SUBMITTER: Ma W 

PROVIDER: S-EPMC7719615 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature