Assessment of Autism Zebrafish Mutant Models Using a High-Throughput Larval Phenotyping Platform.

ABSTRACT: In recent years, zebrafish have become commonly used as a model for studying human traits and disorders. Their small size, high fecundity, and rapid development allow for more high-throughput experiments compared to other vertebrate models. Given that zebrafish share >70% gene homologs with humans and their genomes can be readily edited using highly efficient CRISPR methods, we are now able to rapidly generate mutations impacting practically any gene of interest. Unfortunately, our ability to phenotype mutant larvae has not kept pace. To address this challenge, we have developed a protocol that obtains multiple phenotypic measurements from individual zebrafish larvae in an automated and parallel fashion, including morphological features (i.e., body length, eye area, and head size) and move

SUBMITTER: Colon-Rodriguez A 

PROVIDER: S-EPMC7719691 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature