Ulcerative Colitis-Derived Colonoid Culture: A Multi-Mineral-Approach to Improve Barrier Protein Expression.

ABSTRACT:

Background

Recent studies demonstrated that Aquamin^®, a calcium-, magnesium-rich, multi-mineral natural product, improves barrier structure and function in colonoids obtained from the tissue of healthy subjects. The goal of the present study was to determine if the colonic barrier could be improved in tissue from subjects with ulcerative colitis (UC).

Methods

Colonoid cultures were established with colon biopsies from 9 individuals with UC. The colonoids were then incubated for a 2-week period under control conditions (in culture medium with a final calcium concentration of 0.25 mM) or in the same medium supplemented with Aquamin^® to provide 1.5 - 4.5 mM calcium. Effects on differentiation and barrier protein expression were determined using several app

SUBMITTER: Aslam MN 

PROVIDER: S-EPMC7719760 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature